Immunohistochemical Profiling of Colorectal Tissue

De-identified normal human colorectal tissue samples were obtained from Princeton Cancer Tissue Repository with an IRB approval. Patient consents were obtained at the time of tissue collection. IHC staining was performed as previously described^{14 (link)}. Briefly, tissue sections were deparaffinized in xylene and rehydrated in ethanol and water, followed by antigen retrieval by boiling slides in antigen unmasking solution (Vector Laboratories) for 10 min. Immunostaining was performed using the following antibodies: anti-LIF (Novus, 1:500), anti-lysozyme (Abcam, 1:5000), anti-Olfm4 (Cell signaling, 1:1000), anti-β-catenin (BD, 1:500), anti-CD44 (BioLegend, 1:500), anti-Ki67 (Abcam, 1:200), anti-DCLK (Abcam, 1:200), and anti-cleaved caspase 3 (Cell signaling, 1:500) antibodies.