Viral Nucleic Acid Detection Protocol

Viral nucleic acid from throat swab samples or infected Vero/hSLAM cultures was extracted using the QIAamp Viral RNA/DNA Mini Kit (QIAGEN; Hilden, Germany) according to the manufacturer’s instructions. Viral nucleic acid was detected by real-time PCR/RT-PCR using a detection kit for MeV and rubella virus (RuV) (BioPerfectus Technologies, Taizhou, Jiangsu, China), human herpes virus type 6 (HHV6) and varicella-zoster virus (VZV) (Tianlong Biotechnology Co. Suzhou, Jiangsu, China) according to the manufacturer’s instructions. The detection of other viral febrile respiratory illnesses, such as human cytomegalovirus (CMV)^{29 (link)}, Epstein-Barr virus (EB)^{30 (link)}, human parvovirus B19 (B19)^{31 (link)}, human adenovirus (Adv), human enterovirus (HEV)^{32 (link)} and herpes simplex virus type 1^{33 (link)}, were carried out using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) or Premix Ex Taq™ (Probe qPCR) (TaKaRa; Dalian, China) for RNA or DNA viruses as reported previously.

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Li C., Li Y., Yang Y., Wang J., Zhu C., Tang S., Pang C., Tang W., Cai Q., Li Z., Hu J., Cui X., Zhang X., Xu S., Zhang Y., Yuan Z., Hu Y, & Yuan Z. (2019). The Detection and Characterization of Herpes Simplex Virus Type 1 in Confirmed Measles Cases. Scientific Reports, 9, 12785.

Publication 2019

QIAamp Viral RNA/DNA Mini Kit One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) Premix Ex Taq™ (Probe qPCR)

Dna viruses Enterovirus Epstein barr virus Febrile Herpes simplex virus Human Human adenovirus Human cytomegalovirus Human herpes virus type 6 Human parvovirus b19 Nucleic acid Real time pcr Respiratory Rt pcr Rubella virus Throat Viral dna Viral rna

Corresponding Organization : Shanghai Municipal Center For Disease Control Prevention

Other organizations : Shanghai Public Health Clinical Center, Shanghai Medical College of Fudan University, National Institute for Viral Disease Control and Prevention

Top 5 similar protocols

Variable analysis

independent variables

Viral nucleic acid extraction method (QIAamp Viral RNA/DNA Mini Kit)
Viral nucleic acid detection method (real-time PCR/RT-PCR using detection kits)

dependent variables

Presence/detection of viral nucleic acids (MeV, RuV, HHV6, VZV, CMV, EB, B19, Adv, HEV, HSV-1)

control variables

Throat swab samples or infected Vero/hSLAM cultures
Manufacturer's instructions for viral nucleic acid extraction and detection

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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