A human vocal fold fibroblast cell line produced by the Branski Laboratory, referred to as HVOX, was employed in the current study.1 (link) A primary cell line Human Dermal Fibroblast neonatal (HDFn, ATCC® PCS-201–010) was used for the transcriptomic assay. HVOX and HDFn were cultured in tissue culture plastic flasks and maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Life Technologies) with 10% fetal bovine serum heat-inactivated (Genesee Scientific, San Diego, CA) and 1% penicillin/streptomycin. The media was replaced every 3 days. Cell passages 7–15 were used for this study; HVOX were passaged by incubating with trypsin-EDTA (0.25%, Life Technologies) for 5 minutes and seeding onto tissue culture plastic flasks (VWR, Radnor, PA).
Protocol detail
Characterization of Human Vocal Fold Fibroblasts
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Assay
Cell
Cell line
Eagle
Edta
Fetal bovine serum
Fibroblast
Human
Line
Neonatal
Penicillin
Streptomycin
Tissue
Transcriptomic
Trypsin
Vocal fold
Corresponding Organization : North Carolina State University
Other organizations : New York University
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Variable analysis
independent variables
- Cell line (HVOX and HDFn)
- Transcriptomic assay
- Culture conditions (tissue culture plastic flasks, Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum heat-inactivated and 1% penicillin/streptomycin, media replaced every 3 days)
- Cell passage (7-15)
- Positive control: Not mentioned
- Negative control: Not mentioned
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