Quantifying Thoracic Vertebral Deformation in Mice

Before sacrifice for collection of tissue samples, mice were anesthetized with 0.01/100mg Ketamine® and Xylazine® (4:1) by intraperitoneal route, and fixed in lateral decubitus with the aid of adhesive tape. Digital radiographic images were obtained with the In-vivo Imaging System FX PRO (Carestream Molecular Imaging). To assess the severity of the thoracic vertebra deformation, Kyphosis Index Ratio (KI) was used [7 (link),8 (link)]. Briefly, KI is the ratio between the length of a straight line from the last cervical vertebra to the sixth lumbar vertebra and the length of a line perpendicular to the latter, from the dorsal edge of the vertebra at the point of greatest curvature to the first line.

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de Souza R.B., Farinha-Arcieri L.E., Catroxo M.H., Martins A.M., Tedesco R.C., Alonso L.G., Koh I.H, & Pereira L.V. (2019). Association of thoracic spine deformity and cardiovascular disease in a mouse model for Marfan syndrome. PLoS ONE, 14(11), e0224581.

Publication 2019

In-vivo Imaging System FX PRO

Cervical vertebra Digital radiographic Dorsal vertebra Ketamine Kyphosis Lumbar vertebra Mice Xylazine

Corresponding Organization : Universidade Federal de São Paulo

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Variable analysis

independent variables

Anesthesia (Ketamine® and Xylazine® at 0.01/100mg, 4:1 ratio)

dependent variables

Kyphosis Index Ratio (KI)
Digital radiographic images

control variables

Lateral decubitus position of mice fixed with adhesive tape

controls

No positive or negative controls were explicitly mentioned.

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