Dopamine Quantification in C. elegans

A panspecies dopamine assay kit (Sango Biotech, China) was used to measure total dopamine content in approximately 500 C. elegans. Then, a BCA protein concentration test kit (Beyotime, China) was used to measure the total protein content. The relative level of dopamine secretion in C. elegans was expressed as the ratio of total dopamine content to total protein content, which was significantly comparable between different groups [31 (link), 32 (link)].

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Wang J., Wang D., Setrerrahmane S., Martinez J, & Xu H.M. (2023). The peptide Acein promotes dopamine secretion through clec-126 to extend the lifespan of elderly C. elegans. Aging (Albany NY), 15(24), 14651-14665.

Publication 2023

BCA protein concentration test kit

Corresponding Organization : Institut des Biomolécules Max Mousseron

Other organizations : China Pharmaceutical University

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Variable analysis

independent variables

Manipulation of dopamine levels in C. elegans

dependent variables

Total dopamine content in C. elegans
Total protein content in C. elegans

control variables

Approximately 500 C. elegans used in the experiments

controls

Positive control: Not specified
Negative control: Not specified

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