Inhibitors, JNK-IN-8 (kindly provided by Nathanael S. Gray, Harvard Medical School, Boston, MA; ref. 24 (link)) and batimastat (cat. no.: SML0041; Sigma-Aldrich; ref. 25 (link)) were added directly from 1 mg/mL stock dissolved in DMSO to larval medium preheated to 55–60°C. Same concentration of DMSO was added to controls without inhibitors. The drugs were incubated with the larvae continuously until harvesting at the third instar stage, equivalent to 7 days.
Protocol detail
Pharmacological Inhibition of JNK in Larvae
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Corresponding Organization : University of Liverpool
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Variable analysis
independent variables
- JNK-IN-8 (kindly provided by Nathanael S. Gray, Harvard Medical School, Boston, MA; ref. 24)
- Batimastat (cat. no.: SML0041; Sigma-Aldrich; ref. 25)
- Outcome variables not explicitly mentioned
- Concentration of DMSO
- Positive control: Not specified
- Negative control: Larval medium with the same concentration of DMSO but without inhibitors
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