Cell viability was assessed using a Cell Counting Kit-8 (CCK-8) assay (Apex Bio, TX, USA) following the manufacturer92 (link). Briefly, control or NRBF2-deficient aNSCs were plated in a poly-L-ornithine/laminin-coated 96-well plate at a density of 5000 cells/well and treated with vehicle or bafilomycin A1 (20 nM) for 12 h. Then, CCK-8 was added, and the absorbance at 450 nm was detected using a microplate reader (Tecan, Männedorf, Switzerland).
Other organizations :
Huazhong University of Science and Technology, University of Macau, Ministry of Education of the People's Republic of China, Allen Institute for Brain Science, Icahn School of Medicine at Mount Sinai
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