S. collinus Inha504 Genome Sequencing

The S. collinus Inha504 genome was sequenced at Macrogen (South Korea) using the Illumina HiSeq (Illumina, USA) platforms and the PacBio RSII (Pacific Biosciences, USA). Library preparation for Illumina HiSeq sequencing was performed using the TruSeq DNA sample preparation kit for Illumina (NE, USA), with a library insert size of 350 bp; Library preparation for PacBio RS SMRT sequencing was performed using the PacBio DNA Template Prep Kit 1.0 (Pacific Biosciences, USA) and the library insert size was 20 kb. A high-quality sequence was obtained by correcting the assembled contig error using Pilon (v1.21) software. The de novo assembly of the sequenced fragments was performed using HGAP (v3.0) performs. The validation check of the analyzed fragments was performed using BLAST (v2.7.1 +) software and BUSCO (v3.0) software. The annotation was performed using Prokka (v1.12b) software.