Subcellular Localization of PfbZIP85 Protein

To investigate the subcellular localization of PfbZIP85 protein, the recombinant fusion plasmid 35S::PfbZIP85::GFP (pCAMBIA1300-PfbZIP85/GFP) and the control plasmid 35S::GFP were transiently expressed in N. benthamiana leaf epidermal cells through Agrobacterium-mediated transformation [6 (link)]. After the tobacco plants were grown at 25 °C for more than 36 h, the transfected tobacco leaf samples were treated with DAPI reagent (Solarbio Life Sciences, Beijing, China), followed by observation of fluorescence signals utilizing a laser scanning confocal microscope (Leica TCS SP8).

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Huang X., Zhou Y., Shi X., Wen J., Sun Y., Chen S., Hu T., Li R., Wang J, & Jia X. (2024). PfbZIP85 Transcription Factor Mediates ω-3 Fatty Acid-Enriched Oil Biosynthesis by Down-Regulating PfLPAT1B Gene Expression in Plant Tissues. International Journal of Molecular Sciences, 25(8), 4375.

Publication 2024

DAPI reagent TCS SP8

Corresponding Organization : Shanxi Agricultural University

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Variable analysis

independent variables

Transfection of tobacco leaf epidermal cells with 35S::PfbZIP85::GFP (pCAMBIA1300-PfbZIP85/GFP) fusion plasmid
Transfection of tobacco leaf epidermal cells with 35S::GFP control plasmid

dependent variables

Subcellular localization of PfbZIP85 protein

control variables

Growth of tobacco plants at 25 °C for more than 36 h
Treatment of transfected tobacco leaf samples with DAPI reagent
Observation of fluorescence signals using a laser scanning confocal microscope (Leica TCS SP8)

controls

35S::GFP control plasmid

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