In whole-cell catalysis bioprocess, xylose, erythritol, 1,3-propylene glycol (1,3-PG), and 3-hydroxypropionic acid (3-HPA) were obtained from Aladdin. Xylonic acid (XA) was purchased from TRC-Canada, erythrulose and yeast extract were procured from Sigma. All other chemicals including nutrient salts and sodium alginate were of analytical grade and were commercially available.
The concentration of xylose and XA were detected by high-performance anion-exchange chromatography (HPAEC) coupled with pulsed amperometric detector (Thermo ICS-5000). NaOH (100 mM) was used as mobile phase at flow rate of 0.3 mL/min. The separation column used was CarboPac™ PA200. The titer of erythritol, erythrulose, 1,3-PG and 3-HPA were measured by high-performance liquid chromatography (HPLC) (Agilent 1100 series) equipped with Carbohydrate Ca++ 8um HyperRez XP Column and deionized water, after ultrasound, was used as mobile phase at 0.6 mL/min.
Five parallel assays were performed for each experiment.
The concentration of xylose and XA were detected by high-performance anion-exchange chromatography (HPAEC) coupled with pulsed amperometric detector (Thermo ICS-5000). NaOH (100 mM) was used as mobile phase at flow rate of 0.3 mL/min. The separation column used was CarboPac™ PA200. The titer of erythritol, erythrulose, 1,3-PG and 3-HPA were measured by high-performance liquid chromatography (HPLC) (Agilent 1100 series) equipped with Carbohydrate Ca++ 8um HyperRez XP Column and deionized water, after ultrasound, was used as mobile phase at 0.6 mL/min.
Five parallel assays were performed for each experiment.
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