Rodent Retinal Imaging and Visual Function

The measurements of retinal layers were performed using a Spectralis HRA + OCT device (Heidelberg Engineering, Germany) with several adaptions for rodents.²² The analysis and scanning protocols are described elsewhere^{11 (link),23} and in the eMethods, links.lww.com/NXI/A706, in line with the APOSTEL recommendations.^{24 (link)} Visual function analysis was performed with a testing chamber and OptoMotry software from CerebralMechanics, Canada, as previously described^{23 ,25 (link)} and explained in detail in the eMethods, links.lww.com/NXI/A706.

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Dietrich M., Hecker C., Martin E., Langui D., Gliem M., Stankoff B., Lubetzki C., Gruchot J., Göttle P., Issberner A., Nasiri M., Ramseier P., Beerli C., Tisserand S., Beckmann N., Shimshek D., Petzsch P., Akbar D., Levkau B., Stark H., Köhrer K., Hartung H.P., Küry P., Meuth S.G., Bigaud M., Zalc B, & Albrecht P. (2022). Increased Remyelination and Proregenerative Microglia Under Siponimod Therapy in Mechanistic Models. Neurology® Neuroimmunology & Neuroinflammation, 9(3), e1161.

Publication 2022

Spectralis HRA + OCT device OptoMotry software

Adaptions Device Retinal Rodents Visual function

Corresponding Organization :

Other organizations : Assistance Publique – Hôpitaux de Paris, Sorbonne Université, Institut du Cerveau, Inserm, Heinrich Heine University Düsseldorf, Hôpital Saint-Antoine, University of Sydney, Medical University of Vienna, Centre National de la Recherche Scientifique

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Variable analysis

independent variables

Spectralis HRA + OCT device (Heidelberg Engineering, Germany) with several adaptions for rodents

dependent variables

Measurements of retinal layers
Visual function analysis

control variables

Analysis and scanning protocols described elsewhere and in the eMethods

controls

Positive control: Not mentioned
Negative control: Not mentioned

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