Apoptosis and Mitochondrial Membrane Potential Analysis

Flow cytometric analyzed of apoptosis were using the FITC Annexin V Apoptosis Detection Kit I (BD, USA), and performed as previously described (43 (link)). The cell’s inner mitochondrial membrane potential (Δψm) was detected by flow cytometric using MitoScreen JC-1 staining kit (BD) (44 (link)). Briefly, cells were dissociated with trypsin and resuspended at 1 × 10⁶ cells/ml in Assay Buffer, and then incubated at 37°C for 15 minutes with 10 μl/ml JC-1. Before analyzed by flow cytometer, cells were washed twice by Assay Buffer. Flow cytometry data were analyzed using FlowJo 7.6 software (TreeStar Inc., USA) as previously described (45 (link)).