The ethics committee of the Federal University of Ouro Preto (2015/20) approved all animal experiments, and the experiments were performed according to the rules of animal protection and the ethical principles of the Brazilian Society of the Science in Laboratory Animals (SBCAL).
Forty male C57BL/6 mice (12 weeks old) obtained from the Laboratory of Experimental Nutrition (LABNEX) at the Federal University of Ouro Preto (UFOP) were housed under controlled conditions (12 h light/dark, 21°C ± 2°C, 50% ± 10% humidity) with access to food and water ad libitum. The mice were divided into 5 groups (n = 8 per group): a control group exposed to ambient air (CG), a group that received 200 μL of vehicle solution containing 50% propylene glycol (Sigma Aldrich, Missouri, USA) and 50% saline (VG) by orogastric gavage, a group that received 10 mg/kg/day of quercetin (Sigma Aldrich, Missouri, USA) diluted in 200 μL of propylene glycol solution (QG), a group exposed to cigarette smoke (CSG), and a group administered with quercetin and exposed to cigarette smoke (QCSG). The administration of quercetin was performed via orogastric gavage 1 h before exposure to cigarette smoke [12 (link), 13 (link)].
Forty male C57BL/6 mice (12 weeks old) obtained from the Laboratory of Experimental Nutrition (LABNEX) at the Federal University of Ouro Preto (UFOP) were housed under controlled conditions (12 h light/dark, 21°C ± 2°C, 50% ± 10% humidity) with access to food and water ad libitum. The mice were divided into 5 groups (n = 8 per group): a control group exposed to ambient air (CG), a group that received 200 μL of vehicle solution containing 50% propylene glycol (Sigma Aldrich, Missouri, USA) and 50% saline (VG) by orogastric gavage, a group that received 10 mg/kg/day of quercetin (Sigma Aldrich, Missouri, USA) diluted in 200 μL of propylene glycol solution (QG), a group exposed to cigarette smoke (CSG), and a group administered with quercetin and exposed to cigarette smoke (QCSG). The administration of quercetin was performed via orogastric gavage 1 h before exposure to cigarette smoke [12 (link), 13 (link)].
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