Human PDAC cell lines MIA PaCa-2 and Capan-1 were purchased from ATCC (Manassas, VA, USA). Normal fibroblasts (NFs, id = 6 and 11) were obtained from patients undergoing surgery for PDAC as described previously [9 (link)]. Three pancreatic fibroblasts (PFs, id = 10295, 14289 and 14358) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) (Catalog #3830).
Capan-1 and MIA PaCa-2 were cultured in a Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Palo Alto, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C in 5% CO2. PFs were maintained in a Stellate Cell Medium (ScienCell Research Laboratories, Carlsbad, CA, USA) at 37 °C in 5% CO2, while NFs were maintained in an MF-medium (Toyobo, Tokyo, Japan).
Capan-1 and MIA PaCa-2 were cultured in a Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Palo Alto, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C in 5% CO2. PFs were maintained in a Stellate Cell Medium (ScienCell Research Laboratories, Carlsbad, CA, USA) at 37 °C in 5% CO2, while NFs were maintained in an MF-medium (Toyobo, Tokyo, Japan).
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