Apoptosis was quantified using flow cytometry as detailed in our previous publication (37 (link)), for annexin V binding (BD Biosciences) and 7-aminoactinomycin D (7-AAD) expression (eBioscience). Caspase 3 activity was evaluated by immunoblotting. An MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] proliferation assay was carried out following the manufacturer’s instructions (Promega).
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