Total protein was isolated by using RIPA buffer (Beyotime), and then protein extracts were segregated by using 10% SDS-PAGE. Afterward, the samples were transferred to a PVDF membrane. Western blot was conducted in accordance with previous work [21 (link)]. The primary antibodies against cleaved caspase-3 (ab2302, 1:500), MMP9 (ab228402, 1:1000), GAPDH (ab9485, 1:2500), YY1 (ab109228, 1:1000) and secondary antibodies (ab205718, 1:2000) were all procured from Abcam (Cambridge, MA, USA). Finally, the enhanced chemiluminescence method (Beyotime) was used to measure the protein signals.
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