UN-KPC-961 cells were obtained from S. K. Batra at Eppley Institute for Research in Cancer (Omaha, Nebraska). COLO 205 (CCL-222), HCT15 (CCL-225), HT29 (HTB-38), SW480 (CCL-228), HCT116 (CCL-247), HCT8 (CCL-244), NCI-H508 (CCL-253), HepG2 (HB-8065), Hs746T (HTB-135), CT26 (CRL-2638), LS-174T (CL-88), PANC1 (CRL-1469), and NCI-N87 (CRL-5822) cell lines were purchased from the American Type Culture Collection (ATCC) (Baltimore, MD) and maintained in standard conditions following the providers’ instructions. MC38 cells were purchased from Kerafast (Boston, MA). Lvm3b was generated by in vivo selection from the parental cell line LS-174T (ATCC, Baltimore, MD) (15 (link)). UN-KPC-961 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Langley, OK, 11960-044), 7.5% sodium bicarbonate (Gibco, Langley, OK, 25080-094), 1% penicillin-streptomycin (Lonza, 17-745E), 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, F4135), 200 mM l -glutamine (Gibco, Langley, OK, 25030081), 1 mM Hepes (Gibco, Langley, OK, 15630080), and gentamicin (50 mg/ml) (Gibco, Langley, OK, 15750078). MC38 cells were cultured in DMEM, 10% FBS, and 1 mM Hepes.
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HCT116
HepG2 (HB-8065),
NCI-N87 (
MC38 cells
LS-174T
sodium bicarbonate
penicillin-streptomycin
l-glutamine
Hepes
gentamicin
Cancer
Cell line
Cells
Cultured medium
Eagle
Fetal bovine serum
Gentamicin
Glutamine
Hepes
Ht29
Parental
Penicillin
Sodium bicarbonate
Streptomycin
Corresponding Organization : Rockefeller University
Other organizations : Genome Institute of Singapore, Memorial Sloan Kettering Cancer Center, Sarah Cannon, Mary Crowley Cancer Research Center, City of Hope, University of North Carolina at Chapel Hill, Cedars-Sinai Medical Center, University of Colorado Denver, SUNY Downstate Health Sciences University
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Variable analysis
independent variables
- Cell lines used: UN-KPC-961, COLO 205, HCT15, HT29, SW480, HCT116, HCT8, NCI-H508, HepG2, Hs746T, CT26, LS-174T, PANC1, NCI-N87, MC38, Lvm3b
- Not explicitly mentioned
- Culture conditions for UN-KPC-961 cells: Dulbecco's modified Eagle's medium (DMEM), 7.5% sodium bicarbonate, 1% penicillin-streptomycin, 10% fetal bovine serum (FBS), 200 mM L-glutamine, 1 mM Hepes, and gentamicin (50 mg/ml)
- Culture conditions for MC38 cells: DMEM, 10% FBS, and 1 mM Hepes
- Positive control: None specified
- Negative control: None specified
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