Insulin Amyloid Fibril Inhibition
Corresponding Organization :
Other organizations : Islamic Azad University, Science and Research Branch, University of Tehran, Tehran University of Medical Sciences
Variable analysis
- Presence or absence of 1 mM concentration of silibinin
- Presence or absence of 1 mM concentration of trans-chalcone
- Congo red absorption rate of incubated protein samples
- Final concentration of insulin solution (0.5 mg/mL)
- Phosphate buffer (50 mM, pH 7.4)
- Incubation time (24 h)
- Incubation temperature (37°C)
- Stirring speed (100 rpm)
- Congo red solution (20 μM in 5 mM potassium phosphate and 150 mM sodium chloride, pH 7.4)
- Syringe filter (2-μM pore)
- Spectrophotometer (Shimadzu UV-1800)
- Positive control: Insulin samples incubated in the absence of silibinin and trans-chalcone
- Negative control: Not specified
Annotations
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