Germinated seeds of an extremely late‐bolting genotype ‘NAU‐LB’ were planted in a growth chamber with a photoperiod cycle of 14 h/25 °C light and 10 h/18 °C dark (Xu et al., 2020 (link)). Genomic DNA was extracted from young fresh leaf tissues at the four true‐leaf stage using the DNAsecure Plant Kit (Tiangen Biotech, Beijing, China). A SMRTbell DNA library with 20 kb insertion was constructed using the SMRTbell template prep kit and sequenced on the PacBio Sequel platform (Pacific Biosciences, CA). For BioNano optical mapping, the genomic DNA was labelled with Nb.BssSI and subjected to optical scanning on the BioNano Genomics Saphyr System (Renner et al., 2021 (link)). For Hi‐C sequencing, the isolated DNA from fresh young leaves was fixed, cross‐linked and biotinylated following previous report (Miao et al., 2021 (link)). The libraries of Illumina, 10 × Genomics and Hi‐C sequencing were sequenced on an Illumina HiSeq×Ten platform (Illumina, San Diego, CA).
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Multi-omics Approach for Genome Assembly of Late-bolting Genotype
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Corresponding Organization : Yangzhou University
Other organizations : Nantong University, University of Western Australia
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Variable analysis
independent variables
- Photoperiod cycle: 14 h/25 °C light and 10 h/18 °C dark
- Genomic DNA extraction from young fresh leaf tissues at the four true‐leaf stage
- SMRTbell DNA library construction with 20 kb insertion
- BioNano optical mapping using Nb.BssSI labeling
- Hi‐C sequencing from fresh young leaves
- Growth chamber conditions: Photoperiod cycle, temperature
- No positive or negative controls were explicitly mentioned.
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