Two levels of salinity were selected in the exposure experiment―0 ppt represents freshwater, and 10 ppt represents brackish water. Four days of exposure was determined due to no living larvae observed after 96 h of cultivation with 100 mg/L ZnO NPs under 0 ppt. Differences between treatments are calculated by one-way ANOVA and Tukey’s multiple comparison. Each result was displayed as mean ± standard error (SE), and p < 0.05 was considered as statistically significant. All data analysis and graphs were conducted with SigmaPlot 11.0.
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