In vitro migration assay was performed as we described in the study by Nellius et al.16 (link) with few minor modifications. 1.5 × 106 cells/ml were seeded on top of the gelatinized microporous membrane (8-mm pore size Corning insert). After 2 h, serum-free media was added to the bottom part of the well and incubated at 37 °C overnight. Migrated cells were visualized using the Diff-Quick staining kit (Dade Behring, Deerfield, IL, USA) and counted in 10 high-powered fields ( × 60).
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In vitro Cell Migration Assay
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Corresponding Organization : Texas Tech University
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Variable analysis
independent variables
- Cell seeding density (1.5 × 10^6 cells/ml)
- Cell migration (visualized using Diff-Quick staining and counted in 10 high-powered fields)
- Gelatinized microporous membrane (8-mm pore size Corning insert)
- Serum-free media added to the bottom part of the well
- Incubation at 37 °C overnight
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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