Cell Culture Conditions for KSHV Studies

BJAB-KSHV.219 cells (a kind gift from Thomas Schulz) [52 (link)] were maintained in RPMI media (Corning) supplemented with 10% FBS, 1% penicillin and streptomycin (Corning), 1% L-glutamine (Corning), and 4μg/mL puromycin (Corning). 293-KSHV.219 cells were previously described [24 (link)], and were maintained in DMEM media (Corning) supplemented with 10% FBS, 1% penicillin and streptomycin, 1% L-glutamine, and 1μg/mL puromycin. PEL cells were maintained in RPMI media supplemented with 10% FBS, 1% penicillin and streptomycin, 1% L-glutamine, 0.075% sodium bicarbonate (Corning), and 0.05mM β-mercaptoethanol (Sigma). PEL cells were reactivated with 1 mM NaB (Sigma) and 25 ng/mL TPA (Sigma) where indicated. Vero cells were maintained in DMEM media supplemented with 10% FBS, 1% penicillin and streptomycin, and 1% L-glutamine.