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Transfection and Immunoblotting of ST6 Mutants

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Yao Y., Kim G., Shafer S., Chen Z., Kubo S., Ji Y., Luo J., Yang W., Perner S.P., Kanellopoulou C., Park A.Y., Jiang P., Li J., Baris S., Aydiner E.K., Ertem D., Mulder D.J., Warner N., Griffiths A.M., Topf-Olivestone C., Kori M., Werner L., Ouahed J., Field M., Liu C., Schwarz B., Bosio C.M., Ganesan S., Song J., Urlaub H., Oellerich T., Malaker S.A., Zheng L., Bertozzi C.R., Zhang Y., Matthews H., Montgomery W., Shih H.Y., Jiang J., Jones M., Baras A., Shuldiner A., Gonzaga-Jauregui C., Snapper S.B., Muise A.M., Shouval D.S., Ozen A., Pan K.T., Wu C, & Lenardo M.J. (2022). Mucus sialylation determines intestinal host-commensal homeostasis. Cell, 185(7), 1172-1188.e28.
Publication 2022
Opti-MEM reduced serum media Lipofectamine 3000 Transfection Reagent pLV-EF1a-IRES-Puro
Backbones Cell Co immunoprecipitation Ires Lipofectamine Plasmids Serum Transfection

Corresponding Organization : National Cancer Institute

Other organizations : Frankfurt Cancer Institute, Goethe University Frankfurt, National Institute of Dental and Craniofacial Research, Marmara University, Queen's University, SickKids Foundation, Kaplan Medical Center, Schneider Children's Medical Center, Boston Children's Hospital, National Heart Lung and Blood Institute, Universitätsmedizin Göttingen, Heidelberg University, German Cancer Research Center, Yale University, Howard Hughes Medical Institute, Stanford University, National Eye Institute, Regeneron (United States), Universidad Nacional Autónoma de México, University of Toronto

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