Electroacupuncture Treatment for Experimental Mice

In the EA group, electroacupuncture treatment was performed once a day for 15 days (no treatment on the eight day). The prescription of acupuncture points included DU20 Baihui, DU 26 Shuigou, and EX-HN3 Yintang (the significant extra point). The locations of these points were according to the National Acupuncture Society for Experimental Research developed the “laboratory animal acupuncture atlas”. Huatuo card 30#, 0.5 inch needle was used for treatment. Pricking method was used for DU 26 Shuigou; flat thorn method was used for DU20 Baihui and EX-HN3 Yintang. Needle depth was 0.5 cm and taped. The needle handle was connected with HANS-LH202 electroacupuncture device (Peking University Institute of Science Nerve and Beijing Hua Wei Industrial Development Company), sparse wave, 2 Hz of the frequency, 2 V of the voltage, and 0.6 mA of the current intensity.
In the Rc group and ADc group, do not do any treatment under the same rearing conditions, while grabbing and bondage the mice in order to ensure the same treatment conditions, once a day, 15 days.