Western blotting analysis was performed as previously described.26 (link) The primary antibodies against the following proteins used in the experiment were from commercial sources: anti-JAZF1 (ab80329; 1:200) and anti-p65 (ab16502; 1:2000), both purchased from Abcam; anti-Bcl-2 (#15071; 1:500) and anti-p-p65, both purchased from CST; anti-Bax (ET1603-34; 1:2000) purchased from Hangzhou HuaAn Biotechnology Co., Ltd.; anti-TAK1 (12330-2-AP; 1:500) purchased from Proteintech; anti-GAPDH (SC-365062; 1:800) purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The secondary antibodies were as follows: goat anti-rabbit and goat anti-mouse IgG (1:4000 for both).
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Comprehensive Western Blot Protein Analysis
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Corresponding Organization : Zunyi Medical University
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Variable analysis
independent variables
- Antibodies against the following proteins: anti-JAZF1, anti-p65, anti-Bcl-2, anti-p-p65, anti-Bax, anti-TAK1, anti-GAPDH
- Not explicitly mentioned
- Western blotting analysis was performed as previously described.26 (link)
- Positive controls: not mentioned
- Negative controls: not mentioned
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