Feline Oral Squamous Cell Carcinoma Cell Lines

Feline OSCC lines SCCF1, SCCF2 and SCCF3 were previously established and characterized by one of the investigators (TJR) [27 (link), 28 (link)]. The SCCF1, SCCF2 and SCCF3 cell lines were derived from a laryngeal, bone-invasive gingival and lingual SCC, respectively. Cell lines were maintained in DMEM supplemented with 10 % FBS, non-essential amino acids, sodium pyruvate, penicillin, streptomycin, L-glutamine, and HEPES (4-(2-hydroxythyl)-1-piperazineethanesolfonic acid) at 35 °C, supplemented with 5 % CO₂. LLL12 was synthesized and purified as previously described and provided by Dr. Chenglong Li (College of Pharmacy, The Ohio State University) [24 (link)]. Aliquots of the stock solution were stored at -20 °C until use. The proteasome inhibitor MG132 was purchased from Calbiochem (Billerica, MA). The following antibodies were used for Western blotting and/or immunohistochemistry experiments: p STAT3 (Y705, Cell Signaling Technology, Danvers, MA), STAT3 (Cell Signaling Technology), survivin (Novus Biologicals, Littleton, CO), pAKT (Ser473, Cell Signaling Technology), AKT (BD Biosciences, San Jose, CA) and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA).

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Brown M.E., Bear M.D., Rosol T.J., Premanandan C., Kisseberth W.C, & London C.A. (2015). Characterization of STAT3 expression, signaling and inhibition in feline oral squamous cell carcinoma. BMC Veterinary Research, 11, 206.

Publication 2015

pSTAT3 (Y705, STAT3 survivin pAKT β-actin

Acid Actin Antibodies Biologicals Bone Cell lines Essential amino acids Feline Gingival Hepes Immunohistochemistry L glutamine Laryngeal Lingual Mg132 Novus Penicillin Proteasome inhibitor Pyruvate Sodium Stat3 Streptomycin Survivin

Corresponding Organization :

Other organizations : The Ohio State University

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Variable analysis

independent variables

LLL12 concentration
MG132 (proteasome inhibitor) treatment

dependent variables

PSTAT3 (Y705) expression
STAT3 expression
Survivin expression
PAKT (Ser473) expression
AKT expression

control variables

Cell lines (SCCF1, SCCF2, SCCF3)
Culture conditions (DMEM, 10% FBS, non-essential amino acids, sodium pyruvate, penicillin, streptomycin, L-glutamine, HEPES, 35°C, 5% CO2)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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