Cadmium Imaging in Root Tips

Cd imaging of 1 cm fresh root tips were observed after immersed in Cd probe solution named Leadmium™ Green AM dye (Molecular Probes, Invitrogen, USA) according to Cao et al. method using a laser scanning confocal microscope (TCS SP5, Wetzlar, Germany) with excitation and emission wavelengths of 488 and 515 nm, respectively [3 (link)]. And the fluorescence density of Cd was calculated by selecting the root tips sections in the figures and measuring the total Integrated Density using “Analyse and Measure” function of the Image J software (NIH, Bethesda, MD, USA).

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Sun H., Chen Z.H., Chen F., Xie L., Zhang G., Vincze E, & Wu F. (2015). DNA microarray revealed and RNAi plants confirmed key genes conferring low Cd accumulation in barley grains. BMC Plant Biology, 15, 259.

Publication 2015

Leadmium™ Green AM dye

Fluorescence Laser scanning confocal microscope Molecular probes Root tips

Corresponding Organization :

Other organizations : Zhejiang University, Aarhus University, Yangzhou University

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Variable analysis

independent variables

Cd probe solution named Leadmium™ Green AM dye (Molecular Probes, Invitrogen, USA)

dependent variables

Fluorescence density of Cd

control variables

Fresh root tips
Laser scanning confocal microscope (TCS SP5, Wetzlar, Germany)
Excitation and emission wavelengths of 488 and 515 nm, respectively

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