The LY27805 cell line was kindly provided by Bruno Amati’s group [7 (link)] at the European Institute of Oncology–Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology (IEO–IFOM, Milan, Italy) campus, expanded and stored according to their instructions. Specifically, LY27805 cells were plated at 2 × 105 cells/mL in B cell medium: 50:50 mixture of DMEM and IMDM (Euroclone, Pero (MI), Italy), 10% FBS (Euroclone, Pero (MI), Italy), 2 mM L-glutamine (Euroclone, Pero (MI), Italy), 1% penicillin/streptomycin (Euroclone, Pero (MI), Italy), 50 μM β-mercaptoethanol (Euroclone, Pero (MI), Italy), and non-essential amino acid (NEEA) (Euroclone, Pero (MI), Italy). Phoenix-Ampho cells were cultured with DMEM (Euclone, Pero (MI), Italy), 10%FBS (Euclone, Pero (MI), Italy), 2 mM L-glutamine (Euclone, Pero (MI), Italy), 1% penicillin/streptomycin (Euclone, Pero (MI), Italy).
Cells were tested and authenticated by the StemElite ID System (Promega, Madison, WI, USA). Cells were tested every six months for Mycoplasma by means of the ATCC Universal Mycoplasma Detection Kit 30-1012, cultured for no more than two weeks, and used for no longer than 15 passages.
Cells were tested and authenticated by the StemElite ID System (Promega, Madison, WI, USA). Cells were tested every six months for Mycoplasma by means of the ATCC Universal Mycoplasma Detection Kit 30-1012, cultured for no more than two weeks, and used for no longer than 15 passages.
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