Sensory and Motor Function Assessment in Rats

Before each test, the rats were placed in a Plexiglas box on the mesh grid for a 30 min acclimation period. Mechanical thresholds in rats were tested with von Frey probes (Stoelting, United States) as previously reported (Xu et al., 2018 (link)). Paw withdrawal mechanical threshold (PWMT) was recorded as the force of von Frey filament which induced at least three positive responses out of five applications. Thermal sensitivity thresholds were tested by Hargreaves Tes7370 (Ugo Basile, Italy) (Ding et al., 2017 (link)). Paw withdrawal thermal latency (PWTL) was recorded as the duration of radiant heat stimulation which was turned off automatically by paw withdrawal (cutoff time was 30 s to avoid tissue damage). PWMT and PTWL were measured in triplicate and averaged with an interval of 5 min. The motor function of rats receiving TSA or lentivirus injection was tested at day 10 or 14 after CCI, respectively, using rotarod test following the protocol we have described previously (Dai et al., 2019 (link)).

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Ouyang B., Chen D., Hou X., Wang T., Wang J., Zou W., Song Z., Huang C., Guo Q, & Weng Y. (2019). Normalizing HDAC2 Levels in the Spinal Cord Alleviates Thermal and Mechanical Hyperalgesia After Peripheral Nerve Injury and Promotes GAD65 and KCC2 Expression. Frontiers in Neuroscience, 13, 346.

Publication 2019

Tes7370

Acclimation Filament Lentivirus Plexiglas Rats Sensitivity Tissue

Corresponding Organization : Central South University

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Variable analysis

independent variables

Mechanical thresholds in rats were tested with von Frey probes
Thermal sensitivity thresholds were tested by Hargreaves Tes7370
The motor function of rats receiving TSA or lentivirus injection was tested at day 10 or 14 after CCI, respectively, using rotarod test

dependent variables

Paw withdrawal mechanical threshold (PWMT)
Paw withdrawal thermal latency (PWTL)

control variables

Rats were placed in a Plexiglas box on the mesh grid for a 30 min acclimation period
PWMT and PWTL were measured in triplicate and averaged with an interval of 5 min
Cutoff time for thermal sensitivity test was 30 s to avoid tissue damage

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