Antibodies for Protein Detection Assays

The rabbit anti-Arf1 (1:10,000) and rabbit anti-Drs2 (1:2000) antibodies have been described previously (Chen et al., 1999 (link)). Mouse anti-GFP (1C9A5, 1:2000) and mouse anti-Myc (9E10, 1:2000) antibodies were purchased from the Vanderbilt Antibody and Protein Repository (Nashville, TN). Mouse anti-FLAG M2 (Sigma-Aldrich Cat# F3165, RRID:AB_259529, 1:5000) and mouse anti-HA 12CA5 (Sigma-Aldrich Cat# 11583816001, RRID:AB_514505,1:2500) were purchased from Sigma-Aldrich. Mouse anti-Ubiquitin Ubi-1 antibody (Millipore Cat# MAB1510, RRID:AB_2180556, 1:1000) was purchased from EMD Millipore (Billerica, MA). Mouse anti-Ubiquitin VU1 (LifeSensors Cat# VU101, 1:1000) was purchased from LifeSensors (Malvern, PA). All secondary antibodies, including IRDye 680LT Goat anti-Mouse (LI-COR Biosciences Cat# 827–11080, RRID:AB_10795014, 1:20,000), IRDye 800CW Goat anti-Mouse (LI-COR Biosciences Cat# 827–08364, RRID:AB_10793856, 1:20,000), and IRDye 680LT Goat anti-Rabbit (LI-COR Biosciences Cat# 827–11081, RRID:AB_10795015, 1:20,000), were purchased from LI-COR Biosciences (Lincoln, NE).