Genotyping p75NTR Knockout Mice

We raised p75NTR^−/− mice in the Wenzhou Medical University Animal Laboratory. The differences in body weights and appearances between p75NTR^−/− and p75NTR^+/+ mice were compared. Additionally, we dissected the tail tissue for PCR analysis as previously described [17 (link)]. DNA extraction and PCR amplification were using one step mouse genotyping kit (Vazyme, Nanjing, China) according to the manufacturer’s protocol. The PCR products could be observed on a 3% agarose gel containing 5 mg/mL ethidium bromide.

Free full text: Click here

Shan P., Wang X., Zhang Y., Teng Z., Zhang Y., Jin Q., Liu J., Ma J, & Nie X. (2022). P75 neurotrophin receptor positively regulates the odontogenic/osteogenic differentiation of ectomesenchymal stem cells via nuclear factor kappa-B signaling pathway. Bioengineered, 13(4), 11201-11213.

Publication 2022

one step mouse genotyping kit

Agarose Animal Animal laboratory Ethidium bromide Laboratory mice Mouse P75ntr Tail Tissue

Corresponding Organization : Wenzhou Medical University

Top 5 similar protocols

Variable analysis

independent variables

Genotype (p75NTR−/− vs. p75NTR+/+)

dependent variables

Body weights
Appearances

control variables

Tail tissue samples for PCR analysis
DNA extraction and PCR amplification using one step mouse genotyping kit (Vazyme, Nanjing, China)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!