Inhibitory Compound Acquisition and Characterization

The AZD8055 (Chresta et al., 2010 (link)), Torin2 (Liu et al., 2011 (link)), KU-63794 (García-Martínez et al., 2009 (link)), and WYE-132 (Yu et al., 2010 (link)) were purchased from Selleckchem. PF-4708671 (Pearce et al., 2010 (link)) and allyl/sinigrin were purchased from Sigma-Aldrich. 4MSB and 3MSP GSLs were purchased at C2 Bioengineering. But-3-enyl GSL was purified from Brassica rapa seeds while 3OHPGSL was purified from the aerial parts of 4–5 weeks old greenhouse-grown plants of the Arabidopsis accession Landsberg erecta (Kliebenstein et al., 2001c (link); Crocoll et al., 2016 ). The concentration of 3OHP and but-3-enyl GSL was determined by LC-MS/TQ as desulfo-GSLs. All inhibitors were dissolved in DMSO and stored as 10 mM stocks at –20°C. For allyl, 3MSP, and 4MSB ~ 100 mM GSL stocks were made with H2O and the concentration of GSLs within these stocks was determined by LC-MS/TQ (see above).