sEV morphology was analysed in two sEV samples obtained from plasma and CSF. The morphology was evaluated by transmission electron microscopy (TEM) using the methodology previously described [75 (link),76 (link)]. A volume of 5 µL of resuspended sEVs were fixed in 2.5% glutaraldehyde and then washed with deionised water. Samples were contrasted with 2% uranyl acetate, embedded in 0.13% methyl cellulose and 0.4% uranyl acetate, and then visualised using a Tecnai T20 microscope (FEI Company, Hillsboro, OR, USA), with a filament of LaB6. The voltage used during the visualisation was 200 KV, and acquiring images was performed with a CCD 2 K × 2 K Veleta model (Olympus, Tokyo, Japan).
López-Pérez Ó., Sanz-Rubio D., Hernaiz A., Betancor M., Otero A., Castilla J., Andréoletti O., Badiola J.J., Zaragoza P., Bolea R., Toivonen J.M, & Martín-Burriel I. (2021). Cerebrospinal Fluid and Plasma Small Extracellular Vesicles and miRNAs as Biomarkers for Prion Diseases. International Journal of Molecular Sciences, 22(13), 6822.
Other organizations :
Instituto de Investigación Sanitaria Aragón, Universidad de Zaragoza, Centro de Investigación Biomédica en Red, Biomedical Research Networking Center on Neurodegenerative Diseases, Instituto de Salud Carlos III, Hospital Universitario Miguel Servet, Ikerbasque, CIC bioGUNE, École Nationale Vétérinaire de Toulouse, Institut National Polytechnique de Toulouse
Embedding in 0.13% methyl cellulose and 0.4% uranyl acetate
Visualisation using Tecnai T20 microscope (FEI Company, Hillsboro, OR, USA) with LaB6 filament
Voltage of 200 KV during visualisation
Acquiring images with a CCD 2 K × 2 K Veleta model (Olympus, Tokyo, Japan)
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