Quantitative Analysis of MAP1B, LC3, and PINK1

Quantitative immunoblots of MAP1B were performed as in the previous description of the DM mice [46 (link)], employing the anti-MAP1B antibody from BD Biosciences (San Jose, CA, USA, catalog # 612678), the anti-ACTB antibody from Sigma (Ronkonkoma, NY, USA, # A5441), and the anti-TUBA antibody from Abcam (Cambridge, UK, # ab15246) at the recommended dilutions. Quantitative RT-PCR was carried out with Taqman assays from Thermofisher (Waltham, MA, USA, for PINK1 Hs00260868-m1, for MAP1B Hs00195485-m1, for HPRT1 Hs99999909-m1) again as described in [46 (link)]. Quantitative immunoblots of LC3 isoforms with the anti-LC3 antibody from Sigma (Ronkonkoma, NY, USA, # L8918) and the culture of primary cortical neurons from 1–4-day-old mice with Neurobasal medium over 4 weeks were also done as reported [16 (link)].