Western blot analysis was performed as described previously28 (link), using equal amounts (10 or 50 μg) of protein from each cell lysate. The following antibodies were used: anti-SerpinB2, anti-uPA, anti-LAMP1, anti-phosphorylated p38 (p-p38), anti-p38, anti-phosphorylated ERK (p-ERK), anti-ERK, anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-MMP-2 (Cell Signaling Technology, Danvers, MA, USA); and anti-E-cadherin and anti-N-cadherin (BD Biosciences, San Diego, CA, USA).
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