AT-312 (1-(1-((cis)-4-isopropylcyclohexyl)piperidin-4-yl)-1H-indol-2-yl)methanol) (Figure 1 ) was synthesized at Astraea Therapeutics, and was of >99% chemical purity as fully characterized by nuclear magnetic resonance spectroscopy, LC-MS and elemental analysis. SCH221510 (Figure 1 ) was purchased from Tocris. These test compounds were dissolved in 1-2% DMSO and then diluted to the desired concentration with 0.5% aqueous hydroxypropylcellulose (HPC) and injected subcutaneously (s.c.) in a volume of 0.1 ml/10g of body weight. Controls received 0.1 ml/10g of body weight of the appropriate vehicle (1-2% DMSO in 0.5% of HPC).
Protocol detail
Synthesis and Characterization of AT-312
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Body weight
Dmso
Hydroxypropylcellulose
Methanol
Nuclear magnetic resonance spectroscopy
Sch221510
Therapeutics
Corresponding Organization : Western University of Health Sciences
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Variable analysis
independent variables
- Test compounds: AT-312 and SCH221510
- Not explicitly mentioned
- Concentration of test compounds (1-2% DMSO, 0.5% aqueous hydroxypropylcellulose (HPC))
- Route of administration (subcutaneous injection)
- Volume of administration (0.1 ml/10g of body weight)
- Positive control: Not specified
- Negative control: Vehicle (1-2% DMSO in 0.5% of HPC)
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