Xenopus Oocyte Electrophysiology with Amantadine

Stage V–VI Xenopus laevis oocytes were prepared as described previously (30 (link)). Oocytes injection and TEVC electrophysiological measurements were done as previously described (29 (link)). Amantadine (Sigma, St. Louis, MO) was applied to inhibit A/M2 induced currents. Data were analyzed using ORIGIN 8.0 software (OriginLab, Northampton, MA).

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Balannik V., Wang J., Ohigashi Y., Jing X., Magavern E., Lamb R.A., DeGrado W.F, & Pinto L.H. (2009). Design and Pharmacological Characterization of Inhibitors of Amantadine-Resistant Mutants of the M2 Ion Channel of Influenza A Virus. Biochemistry, 48(50), 11872-11882.

Publication 2009

Amantadine Inhibit Oocytes Xenopus laevis

Corresponding Organization :

Other organizations : Northwestern University, University of Pennsylvania, Institute of Molecular and Cell Biology, Howard Hughes Medical Institute

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Protocol cited in 13 other protocols

Variable analysis

independent variables

Amantadine

dependent variables

A/M2 induced currents

control variables

Stage V–VI Xenopus laevis oocytes
Oocyte injection
TEVC electrophysiological measurements

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