T cells were activated using 2 μM thapsigargin (TG; MilliporeSigma) or with plate-bound mouse anti-human CD3 (10 μg/ml) and mouse anti-human CD28 (10 μg/ml) antibodies (BioLegend) as previously described (21 (link)). In experiments where IFNγ was measured using enzyme-linked immunosorbent assay (ELISA), some cells were activated with phytohemagglutinin (10 μg/ml). The activation protocol and activation times for each experiment are described in the individual figure legends.
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T Cell Activation by Thapsigargin and Anti-CD3/CD28
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Anti cd3
Antibodies
Cells
Enzyme linked immunosorbent assay
Human
Ifnγ
Mouse
Phytohemagglutinin
T cells
Thapsigargin
Corresponding Organization : Cincinnati Children's Hospital Medical Center
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Variable analysis
independent variables
- Thapsigargin (TG; MilliporeSigma) at 2 μM concentration
- Plate-bound mouse anti-human CD3 (10 μg/ml) and mouse anti-human CD28 (10 μg/ml) antibodies (BioLegend)
- Phytohemagglutinin (10 μg/ml)
- IFNγ measured using enzyme-linked immunosorbent assay (ELISA)
- Activation protocol and activation times for each experiment described in the individual figure legends
- Positive control: Phytohemagglutinin (10 μg/ml) for IFNγ ELISA experiments
- Negative control: Not explicitly mentioned
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