Authenticated LNCaP and C4–2B cell lines without mycoplasma contamination were used as described in details in our previous publication[15 (link)]. The KRE (150 mg/mL kavalactones in 50% ethanol) was purchased from Gaia Herbs (Brevard, NC). Main kavalactones, including kawain, 5’,6’-dehydrokawain, yangonin, and methysticin, were isolated and purified by LKT Laboratories, Inc. (St. Paul, MN) from the KRE. Antibodies against MAO-A and tubulin were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Di-methylated histone H3 lysine 4 (H3K4) and H3K9 and Ki67 antibodies were purchased from Abcam (Cambridge, MA). MAO-A and LSD1 activity assay kits were from Cayman Chemical Company (Ann Arbor, Michigan).
Protocol detail
Kavalactones Modulate Epigenetic Markers
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Antibodies against MAO-A and tubulin
Di-methylated histone H3 lysine 4 (H3K4) and H3K9 and Ki67 antibodies
MAO-A and LSD1 activity assay kits
Antibodies
Assay
Cayman
Cell lines
Ethanol
Histone h3
Kawain
Lsd1
Lysine
Mao a
Methysticin
Mycoplasma
Tubulin
Yangonin
Corresponding Organization :
Other organizations : University of California, Irvine
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Variable analysis
independent variables
- KRE (150 mg/mL kavalactones in 50% ethanol)
- MAO-A activity
- LSD1 activity
- Expression levels of di-methylated histone H3 lysine 4 (H3K4) and H3K9
- Ki67 expression
- Authenticated LNCaP and C4–2B cell lines
- Absence of mycoplasma contamination
- Positive control: None mentioned
- Negative control: None mentioned
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