Cell proliferation assay were performed according our previously described, EdU cell proliferation assay kit (Ribobio, Guangzhou, China) was used to detect the effect of BTG1 on cell proliferation [28 (link)]. In a 96-well plate (Guangzhou Jet Bio-Filtration Co., Ltd. Guangzhou, China), 8000 cells were seeded per well. Cellular DNA replication activity was detected by fluorescence microscopy.
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