Cervical Spinal Cord Hemisection in Rats

Rats were anesthetized with an intraperitoneal injection of ketamine HCl (95.0 mg/kg; Vedco), xylazine (10.0 mg/kg; Lloyd Laboratories), and acepromazine (0.075 mg/kg; Phoenix Pharm Inc). Using a #11 surgical blade (Electron Microscopy Sciences), a one-inch midline incision was made on the dorsal surface of skin and muscle to expose the dorsal surface of the C2 and C3 vertebrae. A laminectomy was performed above C2 to expose the spinal cord using rongeurs (Fine Science Tools). A hemisection was performed just caudal to the C2 root with a dissecting knife (Fine Science Tools; Urban et al., 2018 (link)). To ensure a complete hemisection, a 30-gauge needle (BD Biosciences) was passed through the injury several times.

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Urban M.W., Ghosh B., Block C.G., Strojny L.R., Charsar B.A., Goulão M., Komaravolu S.S., Smith G.M., Wright M.C., Li S, & Lepore A.C. (2019). Long-Distance Axon Regeneration Promotes Recovery of Diaphragmatic Respiratory Function after Spinal Cord Injury. eNeuro, 6(5), ENEURO.0096-19.2019.

Publication 2019

ketamine HCl rongeurs dissecting knife 30-gauge needle

Acepromazine Electron microscopy Injury Intraperitoneal injection Ketamine hcl Laminectomy Muscle Needle Rats Root Skin Spinal cord Surgical Vertebrae Xylazine

Corresponding Organization :

Other organizations : Thomas Jefferson University, Temple University, Arcadia, Arcadia University

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Variable analysis

independent variables

Surgical incision on the dorsal surface of skin and muscle to expose the dorsal surface of the C2 and C3 vertebrae
Laminectomy above C2 to expose the spinal cord
Hemisection performed just caudal to the C2 root

dependent variables

Not explicitly mentioned

control variables

Anesthesia: ketamine HCl (95.0 mg/kg), xylazine (10.0 mg/kg), and acepromazine (0.075 mg/kg)
Surgical instruments: #11 surgical blade, rongeurs, dissecting knife, and 30-gauge needle

positive controls

Not specified

negative controls

Not specified

Annotations

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