Seven-day-old 35S:WOX11-SRDX/arf7-1/19-1 and arf7-1/19-1 Arabidopsis seedlings were inoculated with either 90 H. schachtii J2s or a mock solution. Root architecture was inspected at 7 dpi using an Olympus SZX10 binocular with a 1.5× objective and 2.5× magnification. Scans were made of whole seedlings using an Epson Perfection V800 photo scanner. Pictures of nematode infections were taken with an AxioCam MRc5 camera (Zeiss) and the ZEN 3.2 blue edition software (Zeiss).
Nine-day-old 35S:WOX11-SRDX and wild-type Col-0 seedlings, grown on 120 × 120 mm square Petri dishes were inoculated with 0 (mock), 0.5, 1.0, 2.5, 5.0, and 7.5 H. schachtii J2s per milliliter of modified Knop medium as previously described (Guarneri et al. 2023 (link)). Inoculations were done with two 5-µL drops that were pipetted at opposite sides of each seedling while keeping the Petri dishes vertical. At 7 dpi, scans were made of whole seedlings using an Epson Perfection V800 photo scanner. The architecture (i.e. total root length, primary root length, and total secondary root length) was measured using the WinRHIZO package for Arabidopsis (WinRHIZO pro2015, Regent Instrument Inc., Quebec, Canada). The number of root tips was counted manually based on the scans.
Nine-day-old 35S:WOX11-SRDX and wild-type Col-0 seedlings, grown on 120 × 120 mm square Petri dishes were inoculated with 0 (mock), 0.5, 1.0, 2.5, 5.0, and 7.5 H. schachtii J2s per milliliter of modified Knop medium as previously described (Guarneri et al. 2023 (link)). Inoculations were done with two 5-µL drops that were pipetted at opposite sides of each seedling while keeping the Petri dishes vertical. At 7 dpi, scans were made of whole seedlings using an Epson Perfection V800 photo scanner. The architecture (i.e. total root length, primary root length, and total secondary root length) was measured using the WinRHIZO package for Arabidopsis (WinRHIZO pro2015, Regent Instrument Inc., Quebec, Canada). The number of root tips was counted manually based on the scans.
