The procedures for oocyte preparation, cRNA synthesis and injection were identical to those described previously [31 (link)]. cRNA was prepared by in vitro transcription with T7 polymerase using the mMESSAGE mMACHINE high yield capped RNA kit (Ambion, Austin, TX). To enhance the expression of BgNav2-1 and BgNav1-1a and the mutant channels, their cRNAs (2-5 ng/oocyte) were co-injected into oocytes with tipE cRNA from Drosophila melanogaster in a 1:1 ratio [32 (link), 33 (link)]
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Sodium Channel Expression in Oocytes
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Corresponding Organization : Duke University
Other organizations : Tel Aviv University
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Variable analysis
independent variables
- CRNA of BgNa_v2-1 and BgNa_v1-1a and their mutant channels
- Expression level of BgNa_v2-1 and BgNa_v1-1a and their mutant channels
- Procedures for oocyte preparation, cRNA synthesis, and injection
- TipE cRNA from Drosophila melanogaster was co-injected with the cRNAs of the channels and their mutants in a 1:1 ratio as a positive control to enhance the expression of the channels
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