Cardiac Ribo-tag Mouse Model for TAC

All experiments were performed in 9-week-old male mice. The Ribo-tag mice were purchased from Jackson Laboratory (JAX ID 011029). The Ribo-tag mouse was bred to the αMHC-Cre mice or to Cdh5-CreERT2 mouse lines to obtain cardiac myocyte or endothelial Rpl22^HA homozygous mice, respectively. Transverse aortic constriction (TAC) surgery was performed as previously described.^{14 (link)} Animals were randomly assigned to the experimental groups. Sham-operated mice were euthanized at time points matching to TAC surgery time points. Institutional Animal Care and Use Committee approval was obtained for all animal studies.

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Doroudgar S., Hofmann C., Boileau E., Malone B., Riechert E., Gorska A.A., Jakobi T., Sandmann C., Jürgensen L., Kmietczyk V., Malovrh E., Burghaus J., Rettel M., Stein F., Younesi F., Friedrich U.A., Mauz V., Backs J., Kramer G., Katus H.A., Dieterich C, & Völkers M. (2019). Monitoring Cell-Type–Specific Gene Expression Using Ribosome Profiling In Vivo During Cardiac Hemodynamic Stress. Circulation Research, 125(4), 431-448.

Publication 2019

Ribo-tag mice

Animal Aortic constriction Cardiac myocyte Cdh5 Endothelial Homozygous Institutional animal care and use committee Male Mice Surgery

Corresponding Organization :

Other organizations : University Hospital Heidelberg, German Centre for Cardiovascular Research, European Molecular Biology Organization, DKFZ-ZMBH Alliance, German Cancer Research Center

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Variable analysis

independent variables

Genetic manipulation: Breeding Ribo-tag mice to αMHC-Cre mice or Cdh5-CreERT2 mouse lines to obtain cardiac myocyte or endothelial Rpl22^HA homozygous mice, respectively
Surgical intervention: Transverse aortic constriction (TAC) surgery

dependent variables

Outcomes measured at time points matching to TAC surgery time points

control variables

Age of mice: 9 weeks old
Sex of mice: male
Sham-operated mice used as controls for TAC surgery

controls

Positive control: Sham-operated mice
Negative control: Not explicitly mentioned

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