Osteoclastogenesis Assay Protocol

Triton X-100, silver nitrate, naphthol AS-MX phosphate disodium salt, dexamethasone, glycerophosphate, ascorbic acid, 17α-Ethynyl estradiol, and 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Isoflurane was obtained from Aesica Queenborough Limited (Kent, UK). Minimal essential medium alpha (MEM-α) was supplied by Gibco BRL (Grand Island, NY, United States). RANKL was obtained from PeproTech Inc. (Rocky Hill, NJ, United States). 10% formaldehyde solution was purchased from Macron Fine Chemicals (PA, United States).

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Shi B., Lin C.C., Lee C.J., Ning D.S., Lin C.C., Zhao H.W., Yang C.S., Deng S.X., Chiu Y.J, & Wang C.C. (2024). Anti-osteoporotic effects of Yi Mai Jian on bone metabolism of ovariectomized rats. Frontiers in Pharmacology, 15, 1326415.

Publication 2024

Triton X-100 silver nitrate naphthol AS-MX phosphate disodium salt dexamethasone glycerophosphate ascorbic acid 17α-Ethynyl estradiol 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) RANKL

Corresponding Organization :

Other organizations : Infinitus (China)

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Variable analysis

independent variables

Triton X-100
Silver nitrate
Naphthol AS-MX phosphate disodium salt
Dexamethasone
Glycerophosphate
Ascorbic acid
17α-Ethynyl estradiol
3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)

dependent variables

Not explicitly mentioned

control variables

Isoflurane
Minimal essential medium alpha (MEM-α)
RANKL
10% formaldehyde solution

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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