Polyacrylamide gel (12.5%) was used to perform sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Lee et al., 2020 (link)). The extracted myosin samples
(protein concentration, 0.5 mg/mL) were diluted twice (v/v) with sample
buffer (EBA-1051, Elpis Biotech, Daejeon, Korea) and heated at 95°C.
The samples (0.5 mg protein/mL) and protein ladder (3454A, Takara Bio,
Shiga, Japan) were loaded at 10 and 5 μL, respectively. The bands
were stained with a solution containing Coomassie brilliant blue, acetic
acid, and methanol overnight. Then, a buffer containing acetic acid and
methanol was used to de-stain the gels. The gels were scanned at an optical
resolution (63.5 μm/pixel) with a densitometer (GS-710, Bio-Rad
Laboratories) and analyzed using Image Master 2D Platinum v5.0 (GE
Healthcare, formerly Amersham Biosciences, Seoul, Korea).
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Lee et al., 2020 (link)). The extracted myosin samples
(protein concentration, 0.5 mg/mL) were diluted twice (v/v) with sample
buffer (EBA-1051, Elpis Biotech, Daejeon, Korea) and heated at 95°C.
The samples (0.5 mg protein/mL) and protein ladder (3454A, Takara Bio,
Shiga, Japan) were loaded at 10 and 5 μL, respectively. The bands
were stained with a solution containing Coomassie brilliant blue, acetic
acid, and methanol overnight. Then, a buffer containing acetic acid and
methanol was used to de-stain the gels. The gels were scanned at an optical
resolution (63.5 μm/pixel) with a densitometer (GS-710, Bio-Rad
Laboratories) and analyzed using Image Master 2D Platinum v5.0 (GE
Healthcare, formerly Amersham Biosciences, Seoul, Korea).
