Electrocorticography Recording in Awake Rats

ECoG recording was performed as described previously.²⁶ (link), ²⁷ Rats were handled for five consecutive days and acclimatized to a recording chamber prior to the commencement of ECoG recording. ECoG recording occurred in a dimly lit room (0.1 lx) during the dark cycle, which is the active phase in rats.²⁵ (link), ²⁶ (link), ²⁷ , ³⁶ (link) Each awake rat was placed in the transparent electrically shielded plastic box (dimensions 18 × 36 × 30 cm) during acclimation (15 min) and subsequent stimulation sessions. ECoG signals from the electrodes were fed by wire into an amplifier (1000‐fold; DAM80; World Precision Instruments) and band‐pass filtered at 0.1‐1000 Hz. The amplified signals were digitized and recorded at a 4‐kHz sampling rate (Digidata 1200B and Axoscope v1.1; Axon Instruments).