Assessing NF-κB Activity in Endothelial Cells

Transcriptional activity of NF-κB was tested in serum-treated detector endothelial cells by a reporter gene assay, to determine cellular pro-inflammatory effects induced by factors in the sera.^{17 (link)} Transfections in endothelial cells were performed using the Amaxa Nucleofector technology (Amaxa, Gaithersburg, MD), as we have previously reported.^{17 (link)}

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Gardner A.W., Parker D.E., Montgomery P.S., Sosnowska D., Casanegra A.I., Ungvari Z., Csiszar A, & Sonntag W.E. (2015). ENDOTHELIAL CELL INFLAMMATION AND ANTIOXIDANT CAPACITY ARE ASSOCIATED WITH EXERCISE PERFORMANCE AND MICROCIRCULATION IN PATIENTS WITH SYMPTOMATIC PERIPHERAL ARTERY DISEASE. Angiology, 66(9), 867-874.

Publication 2015

Amaxa Nucleofector technology

Assay Cellular Endothelial cells Inflammatory Nf κb Reporter gene Sera Transcriptional Transfections

Corresponding Organization : Veterans Health Administration

Top 5 similar protocols

Variable analysis

independent variables

Serum treatment of endothelial cells

dependent variables

Transcriptional activity of NF-κB in endothelial cells

control variables

Endothelial cell culture conditions
Transfection method (Amaxa Nucleofector technology)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!