Strand-specific libraries were generated as previously described10 (link), from 192 barcoded single-cells and sequenced on a NextSeq Illumina platform. The 192 barcoded single-cell libraries were pooled for sequencing of the HG002 sample. Raw demultiplexed fastq files from the paired-end sequencing run (80-bp read lengths) were uploaded for each single-cell library. These data can be found at https://s3-us-west-2.amazonaws.com/human-pangenomics/index.html?prefix=HG002/hpp_HG002_NA24385_son_v1/Strand_seq/.
Jarvis E.D., Formenti G., Rhie A., Guarracino A., Yang C., Wood J., Tracey A., Thibaud-Nissen F., Vollger M.R., Porubsky D., Cheng H., Asri M., Logsdon G.A., Carnevali P., Chaisson M.J., Chin C.S., Cody S., Collins J., Ebert P., Escalona M., Fedrigo O., Fulton R.S., Fulton L.L., Garg S., Gerton J.L., Ghurye J., Granat A., Green R.E., Harvey W., Hasenfeld P., Hastie A., Haukness M., Jaeger E.B., Jain M., Kirsche M., Kolmogorov M., Korbel J.O., Koren S., Korlach J., Lee J., Li D., Lindsay T., Lucas J., Luo F., Marschall T., Mitchell M.W., McDaniel J., Nie F., Olsen H.E., Olson N.D., Pesout T., Potapova T., Puiu D., Regier A., Ruan J., Salzberg S.L., Sanders A.D., Schatz M.C., Schmitt A., Schneider V.A., Selvaraj S., Shafin K., Shumate A., Stitziel N.O., Stober C., Torrance J., Wagner J., Wang J., Wenger A., Xiao C., Zimin A.V., Zhang G., Wang T., Li H., Garrison E., Haussler D., Hall I., Zook J.M., Eichler E.E., Phillippy A.M., Paten B., Howe K, & Miga K.H. (2022). Semi-automated assembly of high-quality diploid human reference genomes. Nature, 611(7936), 519-531.
Corresponding Organization : Rockefeller University
Other organizations :
National Human Genome Research Institute, National Institutes of Health, Human Technopole, BGI Group (China), Wellcome Sanger Institute, National Center for Biotechnology Information, University of Washington, Dana-Farber Cancer Institute, University of California, Santa Cruz, University of Southern California, James S. McDonnell Foundation, Heinrich Heine University Düsseldorf, University of Copenhagen, Stowers Institute for Medical Research, Illumina (United States), European Molecular Biology Laboratory, Johns Hopkins University, University of California, San Diego, Washington University in St. Louis, Clemson University, Coriell Institute For Medical Research, Material Measurement Laboratory, National Institute of Standards and Technology, Central South University, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Max Delbrück Center, Sun Yat-sen University, Zhejiang University, University of Tennessee Health Science Center, Yale University
Strand-specific libraries were generated from 192 barcoded single-cells
dependent variables
Sequencing of the HG002 sample on a NextSeq Illumina platform
control variables
Control variables not explicitly mentioned
controls
No positive or negative controls were explicitly mentioned in the provided information.
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