For control experiments to compare cytoplasmic/stromal staining approaches, a published MUSE protocol with eosin staining was used.29 (link) In short, tissues were stained with eosin for 2 min and then rinsed in PBS.
Multimodal Tissue Staining Protocol
For control experiments to compare cytoplasmic/stromal staining approaches, a published MUSE protocol with eosin staining was used.29 (link) In short, tissues were stained with eosin for 2 min and then rinsed in PBS.
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of Washington, University of Washington Medical Center
Variable analysis
- SYBR Gold concentration (0.825:10,000 v/v)
- ATTO 655 NHS ester concentration (11.25-μM)
- Eosin concentration (200 μg/ml)
- Nuclear staining (SYBR Gold)
- Stromal/cytoplasmic staining (ATTO 655 NHS ester)
- Cytoplasmic/stromal staining (Eosin)
- Phosphate-buffered saline (PBS) pH 8.0 for staining
- Phosphate-buffered saline (PBS) pH 7.4 for rinsing
- Staining duration (5 min for SYBR Gold and ATTO 655 NHS ester, 2 min for Eosin)
- Rinsing duration (30 s per wash, 3 washes)
- No positive controls mentioned.
- No negative controls mentioned.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!